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Annals of Surgical Oncology 10:171-175 (2003)
© 2003 Society of Surgical Oncology

ORIGINAL ARTICLES

Detection of Bone Marrow Micrometastasis in Gastric Cancer Patients by Immunomagnetic Separation

Katsuhiro Matsunami, MD, Tsutomu Nakamura, MD, Hidetoshi Oguma, MD, Yoichi Kitamura, MD and Ken Takasaki, MD

From the Department of Surgery, Institute of Gastroenterology, Tokyo Women’s Medical University, Tokyo, Japan.

Correspondence: Address correspondence and reprint requests to: Tsutomu Nakamura, MD, Department of Surgery, Institute of Gastroenterology, Tokyo Women’s Medical University, Kawada-cho 8-1, Shinjuku-ku, Tokyo, 162-8666, Japan; Fax: 81-3-5269-7507; E-mail: tsutomu{at}ige.twmu.ac.jp

Background: Micrometastasis to the bone marrow can predict widespread disease and a poor prognosis of cancer patients after surgery. The purpose of this study was to evaluate the clinical significance of detecting micrometastasis in the bone marrow of gastric cancer patients.

Methods: Bone marrow and peripheral blood samples were obtained from 53 gastric cancer patients at the time of surgery. These samples were enriched by immunomagnetic separation and immunostained with an anti-cytokeratin antibody. Expression of vascular endothelial growth factor and erbB-2/HER2 was examined in the primary tumors.

Results: Cytokeratin-positive cancer cells were observed in the bone marrow of 16 (30%) of 53 patients. Among them, two patients also had cancer cells in the peripheral blood. The presence of bone marrow micrometastasis was correlated with the depth of invasion and lymph node metastasis but was not associated with peritoneal dissemination. Detection of bone marrow micrometastasis was not correlated with vascular endothelial growth factor or HER2 expression in the primary tumors. Four patients with micrometastasis had recurrence in the liver or lungs, but this did not occur in patients without micrometastasis.

Conclusions: Detection of cancer cells in the bone marrow might be an indicator of postoperative hematogenous metastasis in gastric cancer patients.

Key Words: Micrometastasis • Cytokeratin • Bone marrow • Immunomagnetic separation • Gastric cancer • Hematogenous metastasis




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S. Gretschel, C. Schick, U. Schneider, L. Estevez-Schwarz, A. Bembenek, and P. M. Schlag
Prognostic Value of Cytokeratin-Positive Bone Marrow Cells of Gastric Cancer Patients
Ann. Surg. Oncol., February 1, 2007; 14(2): 373 - 380.
[Abstract] [Full Text] [PDF]


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