Annals of Surgical Oncology Cite Track
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshinaga, K.
Right arrow Articles by Mori, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshinaga, K.
Right arrow Articles by Mori, M.
Related Collections
Right arrow Cell growth
Annals of Surgical Oncology 10:582-587 (2003)
© 2003 Society of Surgical Oncology

NEW APPROACHES TO THE TREATMENT OF HEPATIC MALIGNANCIES

Platelet-Derived Endothelial Cell Growth Factor Mediates Rho-Associated Coiled-Coil Domain Kinase Messenger RNA Expression and Promotes Cell Motility

Keiji Yoshinaga, PhD, MD, Hiroshi Inoue, PhD, MD, Fumiaki Tanaka, PhD, MD, Koshi Mimori, PhD, MD, Tohru Utsunomiya, PhD, MD and Masaki Mori, PhD, MD

From the Department of Molecular and Surgical Oncology, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.

Correspondence: Address correspondence and reprint requests to: Masaki Mori, MD, PhD, Department of Molecular and Surgical Oncology, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumibaru, Beppu, 874-0838, Japan; Fax: 81-977-27-1651; E-mail: mmori{at}beppu kyushu-u.ac.jp.

ABSTRACT

Background: Platelet-derived endothelial cell growth factor (PD-ECGF), whose expression is increased in several cancers, is an endothelial cell mitogen and has chemotactic activity in vitro and angiogenic activity in vivo. Tumors with high PD-ECGF expression tend to have frequent lymph node metastasis and are associated with poor outcome.

Methods: We screened genes transduced by PD-ECGF transfection to the colon cancer cell line DLD-1 by using a complementary DNA microarray. Cell motility was evaluated by in vitro migration assay. Actin fiber polymerization was visualized by immunofluorescent detection of phalloidin.

Results: Rho-associated coiled-coil domain kinase (ROCK1) was found to be significantly overexpressed in PD-ECGF transfectants compared with mock cells. PD-ECGF transfectants showed higher cell motility than mock cells. The parental cell, DLD-1, with recombinant PD-ECGF showed higher cell motility than that without recombinant PD-ECGF, in which motility was blocked by the neutralizing antibody of PD-ECGF or Y-27632, a specific inhibitor of ROCK1. Moreover, the actin fiber polymerization, which is a marker of activation of ROCK1, was higher in PD-ECGF transfectants than in mock cells.

Conclusions: PD-ECGF expression may be associated with cancer cell migration via activation of ROCK1. This may explain one mechanism by which tumors with high expression of PD-ECGF show aggressive behavior.

Key Words: PD-ECGF • ROCK1 • Cell motility • cDNA microarray




This article has been cited by other articles:


Home page
 Ann. Surg. Oncol.Home page
K. Yoshinaga, K. Yamashita, K. Mimori, F. Tanaka, H. Inoue, and M. Mori
Activin A Causes Cancer Cell Aggressiveness in Esophageal Squamous Cell Carcinoma Cells
Ann. Surg. Oncol., January 1, 2008; 15(1): 96 - 103.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Society of Surgical Oncology.