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Application of Molecular Diagnosis for Detection of Peritoneal Micrometastasis and Evaluation of Preoperative Chemotherapy in Advanced Gastric Carcinoma

From the Department of Surgery and Clinical Oncology, Graduate School of Medicine (TM, YF, YS, TA, TI, KT, ST, TY, MY, MM), Osaka University; and Department of Surgery, Shinsenri Hospital (KY), Osaka, Japan.

Correspondence: Address correspondence and reprint requests to: Yoshiyuki Fujiwara, MD, PhD, Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, 2–2 E2, Yamadaoka, Suita-City, Osaka 565–0871, Japan; Fax: 81-6-6879-3259; E-mail: fujiwara{at}surg2.med.osaka-u.ac.jp

Background: In advanced gastric cancer, peritoneal recurrence is the main cause of death after curative surgical resection. The aim of this report was to describe a novel approach for quantitative genetic diagnosis using peritoneal lavage for the identification of patients at high risk for peritoneal recurrence and for evaluation of the clinical response to intraperitoneal chemotherapy in advanced gastric cancer.

Methods: Nineteen patients with advanced gastric cancer who underwent staging laparoscopy and intraperitoneal chemotherapy before surgical resection or systemic chemotherapy between June 1999 and September 2001 were enrolled in this study. All peritoneal lavage specimens, collected at both staging laparoscopy and gastrectomy, were subjected to real-time quantitative genetic diagnosis.

Results: The reverse transcriptase polymerase chain reaction (RT-PCR) values decreased in 8 cases, stabilized as negative in 5, and increased in 6 during therapy. Patients whose RT-PCR values diminished and were ultimately negative survived except for one, and all but one patient whose values increased during treatment died of recurrence.

Conclusions: Quantitative evaluation of genetic changes can provide accurate, useful information on the effects of preoperative intra-abdominal chemotherapy and overall prognosis for patients with advanced gastric cancer.

Key Words: Gastric cancer • Molecular diagnosis • Peritoneal dissemination • Staging laparoscopy

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