Annals of Surgical Oncology Cite Track
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
10.1245/ASO.2006.05.021
Annals of Surgical Oncology 13:881-886 (2006)
© 2006 Society of Surgical Oncology
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sivarajasingham, N. S.
Right arrow Articles by Monson, J. R. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sivarajasingham, N. S.
Right arrow Articles by Monson, J. R. T.

Original Article

Implication of the BRCA2 and Putative ‘‘BRCA3’’ Genes in Dukes’ Stage C, Replication Error–Negative Colon Cancer

Nala S. Sivarajasingham, MD, Lynn Cawkwell, PhD, Richard P. Baker, MD, Sara L. O’Kane, BSc, Edward F. Smyth, MD, Jonathan V. T. Tilsed, MD, Mark B. Watson, PhD, John Greenman, PhD and John R. T. Monson, MD

Postgraduate Medical Institute of the University of Hull in Association with the Hull-York Medical School, University of Hull, R&D Building, Castle Hill Hospital, Hull, HU16 5JQ, United Kingdom

Correspondence: Address correspondence and reprint requests to: Lynn Cawk-well, PhD; E-mail: l.cawkwell{at}hull.ac.uk.

Background: Although BRCA genes have been implicated in certain tumors, particularly breast tumors, their role in colon tumorigenesis has not been fully explored. We aimed to investigate the association of the BRCA2 and putative ‘‘BRCA3’’ genes in a homogeneous series of right-sided colon cancer specimens.

Methods: Twenty-three Dukes’ stage C, replication error–negative carcinomas were selected from patients with right-sided colon cancer. After histological examination and microdissection, DNA was extracted from normal colon and carcinoma from each patient. Five microsatellite markers spanning the region of BRCA2 and BRCA3 on chromosome 13 (D13S218, D13S219, D13S165, D13S156, and D13S160) and two markers intragenic to BRCA2 and BRCA3 (D13S171 and D13S1308, respectively) were used. Polymerase chain reaction products were analyzed by using a fluorescent allele imbalance assay.

Results: Markers demonstrating the highest allelic imbalance were D13S1308 (53%), D13S171 (33%), and D13S160 (37%).

Conclusions: The intragenic markers D13S1308 (BRCA3) and D13S171 (BRCA2) on chromosome 13 demonstrated a high frequency of allelic imbalance in primary colon carcinoma. This suggests an involvement of BRCA2 and putative BRCA3 in colon tumorigenesis in right-sided, replication error–negative, Dukes’ stage C cancers. Further studies are needed to confirm the precise role of these genes, and any prognostic significance, in colon cancer.

Key Words: BRCA2 • BRCA3 • Colon cancer • Allelic imbalance • Loss of heterozygosity






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Society of Surgical Oncology.