Annals of Surgical Oncology Sign the Guestbook
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

10.1245/s10434-007-9391-y
Annals of Surgical Oncology 14:2896-2902 (2007)
© 2007 Society of Surgical Oncology
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matsuo, T.
Right arrow Articles by Ochi, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Matsuo, T.
Right arrow Articles by Ochi, M.

Original Article

Telomerase Activity in Giant Cell Tumors of Bone

Toshihiro Matsuo, MD, PhD1, Eiso Hiyama, MD, PhD3, Takashi Sugita, MD, PhD4, Shoji Shimose, MD, PhD1, Tadahiko Kubo, MD, PhD1, Yu Mochizuki, MD, PhD1, Nobuo Adachi, MD, PhD1, Kensaku Kojima, MD2, Patrick Sharman, BSc1 and Mitsuo Ochi, MD, PhD1

1 Department of Orthopaedic Surgery, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan
2 Department of Surgery, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan
3 Natural Science Center for Basic Research and Development, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan
4 Department of Orthopaedic Surgery, Hiroshima Prefectural Hospital, 1-5-54 Ujinakanda, Minami-ku, Hiroshima 734-8530, Japan

Correspondence: Address correspondence and reprint requests to: Toshihiro Matsuo, MD, PhD; E-mail: tomatsuo{at}hiroshima-u.ac.jp

Background: A giant cell tumor of bone (GCT) is a histologically benign neoplasma that has an unpredictable pattern of biological aggressiveness. In the present study, we investigated whether there was a correlation between telomere length or the levels of telomerase activity and other clinical features of GCTs, for the possible use of these factors as parameters of aggressiveness or prognosis.

Methods: In 16 surgically resected GCTs specimens, telomere length was assessed by terminal restriction fragments by Southern blot analysis. Telomerase activity was measured by a semiquantitative polymerase chain reaction–based telomeric repeat amplification protocol assay.

Results: Telomere length reduction was observed in 69% of the GCT samples. The telomere lengths of tumors were significantly shorter than those of normal tissue (P = .008). The mean telomere length of grade 3 tumors was significantly shorter than those of grade 1 and 2 tumors (P = .038). Telomerase activity was detected in 81% of tumor samples. The level of telomerase activity in tumors with local recurrence was significantly higher than in tumors without local recurrence (P = .011).

Conclusions: These results suggest that telomere length correlates with roentgenographic grade as a result of the frequency of cell division, and high telomerase activity indicates the aggressiveness of GCTs.

Key Words: Telomere length • Telomerase activity • Giant cell tumor of bone • Clinical marker • Aggressiveness




This article has been cited by other articles:


Home page
JBJSHome page
T. Matsuo, J. W. Shay, W. E. Wright, E. Hiyama, S. Shimose, T. Kubo, T. Sugita, Y. Yasunaga, and M. Ochi
Telomere-Maintenance Mechanisms in Soft-Tissue Malignant Fibrous Histiocytomas
J. Bone Joint Surg. Am., April 1, 2009; 91(4): 928 - 937.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Society of Surgical Oncology.