Annals of Surgical Oncology Sign the Guestbook
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
10.1245/s10434-007-9564-8
Annals of Surgical Oncology 14:3419-3427 (2007)
© 2007 Society of Surgical Oncology
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Higashiyama, M.
Right arrow Articles by Shimada, Y.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Higashiyama, M.
Right arrow Articles by Shimada, Y.

Original Article

Prognostic Significance of Osteopontin Expression in Human Gastric Carcinoma

Motoshige Higashiyama, MD1, Tetsuo Ito, MD, PhD2, Eiji Tanaka, MD, PhD1 and Yutaka Shimada, MD, PhD, FACS3

1 Department of Surgery, Kyoto University, Kawaracho Shogoin Sakyo-ku, Kyoto, 606-8507, Japan
2 Division of Gastroenterology, Department of Medicine, Johns Hopkins School of Medicine, 1503 E Jefferson St. Rm 106, Baltimore, MD 21231, USA
3 First Department of Surgery, Hyogo College of Medicine, Mukogawa-cho 1-1, Nishinomiya, 663-8501, Japan

Correspondence: Address correspondence and reprint requests to: Yutaka Shimada, MD, PhD, FACS; E-mail: sshimada{at}hyo-med.ac.jp

Background: Osteopontin (OPN) is a secreted, integrin-binding glycophosphoprotein that has been implicated in the progression of various solid tumors. To evaluate the clinical significance of OPN in gastric carcinoma, we investigated OPN expression in resected tumors.

Methods: Expression of OPN protein by gastric cancer cells was evaluated using western blot analysis. OPN messenger RNA (mRNA) expression in 18 gastric cancers was compared with that in the corresponding normal gastric epithelium by semiquantitative reverse transcription polymerase chain reaction (RT-PCR). Paraffin sections of tumors from 295 patients with gastric cancer were also investigated using immunohistochemistry.

Results: All four gastric cancer cell lines analyzed using western blotting had almost the same level of OPN protein expression as the positive control (HeLa cells). OPN mRNA was upregulated in 83% (15/18) of the tumors studied. On immunohistochemical staining, 90 tumors were classified as negative (–), whereas 205 were classified as positive (1+, 2+, or 3+). The level of OPN protein expression was significantly associated with the patient’s age (p = 0.04), tumor depth (p = 0.03), histological grade (p = 0.008), and hematogenous metastasis (p = 0.007). Kaplan–Meier analysis showed that OPN positivity was significantly associated with a shorter survival time (p = 0.027). Furthermore, multivariate analysis revealed that OPN positivity was an independent risk factor for hematogenous metastasis (p = 0.034).

Conclusions: The present findings suggest that increased tumor expression of OPN is an important determinant of shorter survival time and that OPN positivity may be useful for predicting the risk of hematogenous metastasis in gastric cancer patients.

Key Words: Osteopontin • Hematogenous metastasis • Peritoneal dissemination • Overall survival • Gastric adenocarcinoma




This article has been cited by other articles:


Home page
 J. Thorac. Cardiovasc. Surg.Home page
Z. T. Hammoud, S. Badve, Q. Zhao, L. Li, R. Saxena, M. A. Thorat, A. Morimiya, K. M. Rieger, and K. A. Kesler
Differential gene expression profiling of esophageal adenocarcinoma
J. Thorac. Cardiovasc. Surg., April 1, 2009; 137(4): 829 - 834.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Society of Surgical Oncology.