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10.1245/s10434-007-9363-2
Annals of Surgical Oncology 14:1880-1889 (2007)
© 2007 Society of Surgical Oncology
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Original Article

{alpha}1,2Fucosylation Is a Superior Predictor of Postoperative Prognosis for Colorectal Cancer Compared with Blood Group A, B, or Sialyl Lewis X Antigen Generated within Colorectal Tumor Tissues

Kaori Tsuboi, MD1, Takayuki Asao, MD, PhD1, Munenori Ide, MD, PhD1, Shinji Hashimoto, MD1, Kasumi Noguchi, PhD2, Yoshihiko Kominato, MD, PhD3, Abby R. Saniabadi, PhD4, Hiroyuki Kuwano, MD, PhD1 and Shin Yazawa, PhD1,5,6

1 Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, 371-8511, Japan
2 Criminal Investigation Laboratory, Gunma Prefectural Police HQ, Maebashi, 371-0846, Japan
3 Department of Legal Medicine and Molecular Genetics, Graduate School of Medicine, Gunma University, Maebashi, 371-8511, Japan
4 Japan Immunoresearch Laboratories, Takasaki, 370-0021, Japan
5 Tokushima Research Institute, Otsuka Pharmaceutical Co. Ltd., Tokushima, 771-0192, Japan
6 Innovation Center for Cooperative Research of Gunma University, Maebashi, 371-8511, Japan

Correspondence: Address correspondence and reprint requests to: Shin Yazawa, PhD; E-mail: syazawa{at}titan.ocn.ne.jp

Background: We have previously demonstrated tumor-specific {alpha}1,2fucosylation, which is associated with resistance of tumor cells to anticancer treatment in human colorectal tumor tissues. By using the YB-2 monoclonal antibody, the resulting products have been identified as Y, Leb, and H type 2 antigens in colorectal tumor tissues.

Methods: Immunohistochemical analyses of colorectal cancer tissues (74 specimens) were performed with a newly established mouse monoclonal antibody, YB-3 specifically recognizing H disaccharide (Fuc{alpha}1,2Galß) structures, and anti-A, anti-B, YB-2, and anti–sialyl Lewis X (SLX) antibodies, together with the analyses of glycosyltransferases involved in the synthesis of ABH antigens in the same tissues.

Results: The YB-3 antibody enabled us to detect colorectal tumors, particularly tumors in the distal large intestine and the rectum, with high sensitivity (74.3%) and specificity (100%). From immunohistochemical and enzymatic analyses of colorectal tissues, we found that once {alpha}1,2fucosylation had proceeded in tumor tissues, blood group A or B antigen was also synthesized in approximately half of the tissues of A or B blood type, but not in their normal tissues. A correlation of survival rate with immunostaining of tissues was found only by YB-3 antibody and not by anti-A, anti-B, or anti-SLX antibody.

Conclusions: As a predictor of postoperative prognosis of patients with colorectal cancer, immunodetection of {alpha}1,2fucosylated antigens with the YB-3 antibody seemed to be superior to blood groups A, B, or SLX antigen in colorectal tumor tissues.

Key Words: Colorectal cancer • {alpha}1,2Fucosylation • Distal colon • Blood group A and B antigens • Sialyl Lewis X antigen • A and B enzyme • Survival rate • Diagnosis






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