| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Annals of Surgical Oncology, Vol 5, Issue 7 627-634, Copyright © 1998 by Society of Surgical Oncology
ARTICLES |
G. Aue, B. Muralidhar, H. S. Schwartz and M. G. Butler
Department of Orthopaedics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2550, USA.
BACKGROUND: Telomerase is a ribonucleoprotein that adds TTAGGG nucleotide repeats onto the ends of eukaryotic chromosomes to maintain telomere integrity. Somatic cells do not express telomerase and stop dividing when the chromosomal ends are shortened critically after many cell divisions. Immortal cell lines and cancer cells apparently have telomerase activity that contributes to an unlimited number of cell cycles. The purpose of our study is to investigate whether telomerase activity is expressed in primary malignant tumors of the skeletal system when compared to adjacent normal tissue. METHODS: Fresh tumor and normal tissue was collected from 14 patients (10 males, 4 females; age range, 8 to 76 years) and protein extraction performed. The tumors included seven osteosarcomas (three examined before and after chemotherapy), two chondrosarcomas, two spindle cell tumors, one hemangiopericytoma, one chordoma, and one adamantinoma. Telomerase activity was analyzed by using a highly sensitive polymerase chain reaction (PCR)-based assay (telomere repeat amplification protocol [TRAP]). RESULTS: Telomerase activity was found in 8 of 14 sarcoma patients (57%) using the TRAP assay. Compared to HeLa cell extract (positive control), telomerase activity in the tumor specimen ranged from 0 (in osteosarcoma) to 11.7% (in hemangiopericytoma). There was variation in the number of telomeric repeats generated by telomerase. At least five telomeric bands (e.g. 50, 56, 62, 68, 74 bp) in a ladder pattern had to be present before telomerase activity was considered positive in our analysis. CONCLUSIONS: Telomerase activity may be an oncogenic sustaining event helping to maintain the transformed phenotype seen in malignant tumors of the bone. The degree of telomerase activity varies among skeletal malignancies, but was less than that observed in HeLa cells. The majority of osteosarcomas showed no telomerase activity.
This article has been cited by other articles:
 |
|
 |
|
  T. Matsuo, E. Hiyama, T. Sugita, S. Shimose, T. Kubo, Y. Mochizuki, N. Adachi, K. Kojima, P. Sharman, and M. Ochi Telomerase Activity in Giant Cell Tumors of Bone Ann. Surg. Oncol., October 1, 2007; 14(10): 2896 - 2902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Marina, M. Gebhardt, L. Teot, and R. Gorlick Biology and Therapeutic Advances for Pediatric Osteosarcoma Oncologist, July 1, 2004; 9(4): 422 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Gorlick, P. Anderson, I. Andrulis, C. Arndt, G. P. Beardsley, M. Bernstein, J. Bridge, N.-K. Cheung, J. S. Dome, D. Ebb, et al. Biology of Childhood Osteogenic Sarcoma and Potential Targets for Therapeutic Development: Meeting Summary Clin. Cancer Res., November 15, 2003; 9(15): 5442 - 5453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Martin and J. A. Buckwalter Telomere Erosion and Senescence in Human Articular Cartilage Chondrocytes J. Gerontol. A Biol. Sci. Med. Sci., April 1, 2001; 56(4): 172B - 179. [Abstract] [Full Text]
|
|
|
|
|
|
K. Aogi, A. Woodman, V. Urquidi, D. C. Mangham, D. Tarin, and S. Goodison Telomerase Activity in Soft-Tissue and Bone Sarcomas Clin. Cancer Res., December 1, 2000; 6(12): 4776 - 4781. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
